RESUMO
This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment. Following background presentations about current practice, the expert panel developed a set of consensus points to define the minimum criteria for oocyte and embryo morphology assessment. It is expected that the definition of common terminology and standardization of laboratory practice related to embryo morphology assessment will result in more effective comparisons of treatment outcomes. This document is intended to be referenced as a global consensus to allow standardized reporting of the minimum dataset required for the accurate description of embryo development. This paper reports the proceedings and outcomes of an international consensus meeting on human oocyte and embryo morphology assessment. An expert panel developed a series of consensus points to define the minimum criteria for such assessments. The definition of common terminology, and standardization of laboratory practices related to these morphological assessments, will permit more effective comparisons of treatment outcomes around the world. This report is intended to be referenced as a global consensus to allow standardized reporting of the minimum descriptive criteria required for routine clinical evaluations of human embryo development in vitro.
Assuntos
Embrião de Mamíferos/citologia , Desenvolvimento Embrionário/fisiologia , Recuperação de Oócitos/normas , Oócitos/citologia , Técnicas de Reprodução Assistida/normas , Blastômeros/citologia , Aberrações Cromossômicas , Fase de Clivagem do Zigoto/classificação , Humanos , Oócitos/classificação , Terminologia como Assunto , Resultado do TratamentoRESUMO
BACKGROUND: Many variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment. METHODS: Background presentations about current practice were given. RESULTS: The expert panel developed a set of consensus points to define the minimum criteria for oocyte and embryo morphology assessment. CONCLUSIONS: It is expected that the definition of common terminology and standardization of laboratory practice related to embryo morphology assessment will result in more effective comparisons of treatment outcomes. This document is intended to be referenced as a global consensus to allow standardized reporting of the minimum data set required for the accurate description of embryo development.
Assuntos
Embrião de Mamíferos/citologia , Desenvolvimento Embrionário/fisiologia , Recuperação de Oócitos/normas , Oócitos/citologia , Técnicas de Reprodução Assistida/normas , Blastômeros/citologia , Aberrações Cromossômicas , Fase de Clivagem do Zigoto/classificação , Humanos , Oócitos/classificação , Terminologia como Assunto , Resultado do TratamentoRESUMO
Oocytes with three or more pronuclei and one or two polar bodies arising after intracytoplasmic sperm injection were analyzed cytogenetically, and the data were used to develop a general model explaining the possible origin of the abnormal pronuclear stages. The mechanisms, either separately or in variable combinations, that induce the appearance of additional pronuclei are: 1) nonextrusion of the second polar body; 2) incomplete chromatid segregation into the extruded second polar body; and 3) dispersal of the oocyte chromatids in the presence of the second polar body formation.
Assuntos
Fase de Clivagem do Zigoto , Formação de Conceito , Análise Citogenética/métodos , Oócitos , Injeções de Esperma Intracitoplásmicas , Aneuploidia , Núcleo Celular/fisiologia , Divisão do Núcleo Celular/fisiologia , Células Cultivadas , Segregação de Cromossomos/fisiologia , Fase de Clivagem do Zigoto/classificação , Fase de Clivagem do Zigoto/metabolismo , Fase de Clivagem do Zigoto/fisiologia , Formação de Conceito/fisiologia , Feminino , Fertilização/genética , Humanos , Masculino , Oócitos/citologia , Oócitos/metabolismo , Oócitos/fisiologia , Projetos Piloto , Estudos RetrospectivosRESUMO
New Current Procedural Terminology (CPT) codes were adopted in 2004 for utilization with assisted reproductive technologies.
Assuntos
Técnicas de Laboratório Clínico/normas , Prontuários Médicos/normas , Técnicas de Reprodução Assistida , Fase de Clivagem do Zigoto/classificação , Criopreservação/métodos , Técnicas de Cultura Embrionária , Transferência Embrionária , Feminino , Líquido Folicular/fisiologia , Controle de Formulários e Registros , Humanos , Masculino , Oócitos/citologia , Análise do Sêmen/métodos , Recuperação Espermática , Preservação de Tecido/métodosRESUMO
The policy of single embryo transfer (SET) adopted for women <36 years old since 1 July 2003, strongly calls for improvement of embryo selection. A total of 196 cycles in which SET was performed were randomly allocated to two groups. In the first group, early cleavage was assessed (ECA) 25 h after insemination. The embryo with the best score that cleaved early, if present, was selected for transfer. In the second group, early cleavage was not assessed (ECNA) and embryo selection was based solely on the embryo score. Ninety-eight cycles were allocated in the ECA and ECNA group respectively. Early cleavage occurred in 64% of cycles and 32.2% of embryos. Patient population and embryo morphology were similar between the two groups, and similar delivery rates were observed (27.6 versus 24.5% respectively in the ECA and ECNA groups). The assessment of early cleavage as additional parameter did not improve the delivery rate in the single embryo transfer policy.
